Fluorous Technologies is a unit of Boron Specialties, LLC, 2301 Duss Avenue Suite 35, Ambridge, PA 15003

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FluoroFlash® Silica Gel

Bulk FluoroFlash® silica gel is available in two particle sizes, 40 and 5.  The 40 gel is suitable for use in SPE cartridges and multi-well SPE plates (1).  The 5 gel is recommended for proteomics and bioreagent applications where nanocapillary columns are required (2).  Quantities can be custom-packaged to your needs.

1. Zhang, W.; Lu, Y.; Nagashima, T. J. Comb. Chem. 2005, 7, 893-897.

2. Brittain, S. M.; Ficarro, S. B.; Brock, A.; Peters, E. C. Nature Biotechnology, 2005, 23, 463-468.


Please contact us for pricing on the products below.

PRODUCT -  SILICA GEL

CATALOG NUMBER

FluoroFlash® Silica Gel, 40 µm (25g pkg)

801-0025B

FluoroFlash® Silica Gel, 40 µm (100g pkg)

801-0100B

FluoroFlash® Silica Gel, 40 µm (1 kg)  

801-1000B

FluoroFlash® Silica Gel, 5 µm (1g pkg)

803-1100B

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FluoroFlash® Thin-Layer Chromatography plates


PRODUCT -  TLC PLATES

CATALOG NUMBER

FluoroFlash® TLC Plates 10 pack, 5x10cm, F254 indicator

803-9510PF

FluoroFlash® glass slides:  Fluorous Immobilization for Microarray Formation

In addition to separations, the unique properties of fluorous compounds allow them to be immobilized onto fluorous modified glass surfaces in the formation of microarrays.  Prof. Nicola Pohl at Iowa State University first demonstrated that microarrays could be formed using a fluorous modified slide by spotting fluorous tagged carbohydrates and detecting specific carbohydrate-lectin interactions. [1] Recently, Prof. Pohl has expanded the fluorous microarray work to the quantitative evaluation of lectin-carbohydrate binding and the elucidation of new lectin binding motifs. [2] Based on this seminal work, fluorous small molecule microarrays were then produced independently by Prof. David Spring and co-workers at the University of Cambridge [3] and Prof. Stuart Schreiber and co-workers at the Broad Institute of MIT and Harvard.[4]  The latter publication validated the fluorous microarray data by comparing the results over three different enzymes with that found using biochemical assays and SPR.  Given the number of molecular classes that have been synthesized using fluorous techniques, it is highly likely that these other classes can also be immobilized in microarrays.

 Fluorous based microarrays feature:

– excellent spot morphology

– exceptionally high signal-to-noise ratios

– low and uniform background fluorescence levels

– low non-specific binding

–simpler workflows through the minimization of blocking and washing steps

Fluorous chemistry is the only strategy which allows synthesis, purification, and immobilization using a single tag in a streamlined process with no other chemical manipulations.  One can go directly from chemical reactions to purification to microarray formation linking high quality content generation with high quality assay results.

Ko, K.S.; Jaipuri, F.A.; Pohl, N.A. “Fluorous-Based Carbohydrate Microarrays” J. Am. Chem. Soc. 2005, 127, 13162-13163.

Jaipuri, F.A.; Collet, B.Y.M.; Pohl, N.L., “Synthesis and Quantitative Evaluation of Glycero-D-manno-Heptose Binding to Concanavalin A by Fluorous-Tag Assistance” Angewandte Chemie International Edition 2008, DOI: 10.1002/anie.200704262.

Nicholson, R.L.; Ladlow, M.L.; Spring, D. "Fluorous tagged small molecule microarrays" Chem. Commun., 2007, 38, 3906.

Vegas, A.J.; Bradner, J.E.; Tang, W.; McPherson, O.M.; Greenberg, E.F.; Koehler, A.N.; Schreiber, S.L. “Fluorous-Based Small-Molecule Microarrays for the Discovery of Histone Deacetylase Inhibitors” Angewandte Chemie International Edition 2007, 46, 42, 7960.

PRODUCT - GLASS SLIDES

CATALOG NUMBER

Fluorous Modified Glass Slides  (25x75x1.0mm fluorous modified microarray glass slides)

850-9200