Tag Archives: Proteomics

Isotope-coded Fluorous PALs

Prof. Qisheng Zhang at UNC-Chapel Hill has published several papers about the synthesis and use of fluorous tagged photoaffinity labels (PALs) and has just published another report in Chemical Communications.  The general strategy is to attach a photosensitive reactive group … Continue reading

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Biomolecule Labeling: Fluorous Luminescence

The labeling of biomolecules with exogenous reagents is generally done for one of two purposes either enrichment or detection.  Enrichment labeling is generally in the form of some sort of affinity tag such as biotin, a His tag, and yes, … Continue reading

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Fluorous Photoaffinity Labeling

Photoaffinity labeling reagents is a valuable way by which to study the interactions between two molecular entities.  Often times this is a ligand and it’s receptor.  The strategy is to attach the photolabel to a ligand, incubate with it’s binding … Continue reading

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Fluorous Enrichment in Chemical Genetics and Kinases

One of the primary observations that has come out of the sequencing of the human genome is that the estimated number of genes is much lower than initially anticipated and is somewhere in the 20,000-25,000 range.  This means that the … Continue reading

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Fluorous Enrichment for PTM Detection

One of the challenges in proteomics is the detection and quantitation of low level peptide species.  Often times these are specific posttranslational modifications (PTMs) of proteins which are present only in small amounts but can have big biological effects.  As … Continue reading

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