Category Archives: Proteomics

Fluorous Enrichment in Chemical Genetics and Kinases

One of the primary observations that has come out of the sequencing of the human genome is that the estimated number of genes is much lower than initially anticipated and is somewhere in the 20,000-25,000 range.  This means that the … Continue reading

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Fluorous Enrichment for PTM Detection

One of the challenges in proteomics is the detection and quantitation of low level peptide species.  Often times these are specific posttranslational modifications (PTMs) of proteins which are present only in small amounts but can have big biological effects.  As … Continue reading

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Fluorous Proteomics: Amine Tagging

Since the introduction of fluorous techniques in protoemics in 2005 by Eric Peters and co-workers at the Genomics Institute of the Novartis Research Foundation, several groups have used fluorous methods for the enrichment of specific peptide subsets.  Fluorous methods represent … Continue reading

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Glycosphingolipid Analysis using Fluorous Tags

Glycosphingolipids (GSLs) are an important class of molecules which have been associated with several cellular functions including antigen activity, cell adhesion, and signal transduction.  As the name suggests these molecules consist of a glycan portion and a lipid portion.  Study … Continue reading

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Fluorous Photoaffinity Labeling

Late last year Prof. Qisheng Zhang at the University of North Carolina published a paper describing his group’s efforts at developing fluorous diazirines as photoaffinity labeling reagents.  For more details on Prof. Zhang’s work, you can view the presentation he … Continue reading

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