Fluorous solid phase extraction (F-SPE) is used for quick separations of reaction mixtures involving fluorous catalysts, reagents, protecting groups, tags, and scavengers (1-6). F-SPE is used for quick separations of reaction mixtures involving FluoroFlash® tags, reagents, scavengers and catalysts. The separation of fluorous compounds by F-SPE is a reliable and generic procedure that more greatly resembles filtration than chromatatography. The facile separation depends primarily on the presence or absence of a light fluorous tag, not polatity or other molecular features that control traditional chromatography.
A simple two-step separation of fluorous compounds involves the loading of a reaction mixture containing the fluorous-tagged molecule on to an F-SPE cartridge, washing the non-fluorous compounds away using a fluorophobic wash, then washing the fluorous compounds with a fluorophilic wash. Please consult the F-SPE application note for a more detailed procedure.
FluoroFlash® SPE cartridges are pre-packed in a variety of formats with a proprietary fluorous silica gel. A Fluorous Dye Separation Kit (cited on the F-SPE Application Note) is available to demonstrate how the F-SPE works. If you require a particular format, i.e. specific cartridge size, multi-well plate, etc., please contact FTI.
1. Zhang, W.; Curran, D. P. Tetrahedron 2006, 62, 11837–11865.
2. Curran, D. P. Handbook of Fluorous Chemistry, 2004, 101-155.
3. Zhang, W.; Curran, D. P.; Chen, C. H.-T. Tetrahedron, 2002, 58, 3871-3875.
4. Curran, D.; Lou, Z. Y. Green Chem. 2001, 3, G3-G7.
5. Zhang, Q.; Luo, Z.; Curran, D. P. J. Org. Chem. 2000, 65, 8866-8873.
6. Curran, D. P.; Hadida, S.; He, M. J. Org. Chem. 1997, 62, 6714-6715.
|
