F-Msc-Cl is a base labile fluorous tag based on the Msc (methylsulfonylethoxycarbonyl) protecting group used for the N-protection of amines. Overkleeft used the F-Msc group in the N-terminal tagging of peptides synthesized using a Fmoc based SPPS strategy (1). The F-Msc tagged peptides were purified by either F-HPLC or F-SPE. The F-Msc group was removed by treatment with piperidine to provide the final peptide.
1. de Visser, P. C.; van Helden, M.; Filippov, D. V.; van der Marel, G. A.; Drijfhout, J. W.; van Boom, J. H.; Noort, D.; Overkleeft, H. S. Tetrahedron Lett. 2003, 44, 9013-9016.